Document Type : Original Article


1 Department of Microbiology, Mayo Institute of Medical Sciences, Barabanki, Uttar Pradesh, India

2 Department of Physiology, Mayo Institute of Medical Sciences, Barabanki, Uttar Pradesh, India


Objective: Methicillin‑resistant Staphylococcus aureus (MRSA) ranks top among the 
nosocomial pathogens. Nasal formulation of mupirocin is found to eradicate MRSA from 
colonized individuals, but the emergence of resistant strains is a matter of concern.
Methods: Nasal swabs were collected from 200 health care workers (HCWs) who were 
screened for MRSA. Kirby–Bauer disc diffusion method was used to perform antibiotic 
susceptibility test. MRSA detection was done using a cefoxitin 30 µg disc and interpreted 
according to the Clinical and Laboratory Standards Institute guidelines. Determination of 
mupirocin resistance was performed using Epsilometer test (E‑test).
Findings: About 14% of HCWs showed nasal carriage of MRSA. Nursing orderlies were 
the predominant carriers. E‑test showed four mupirocin resistant isolates. The antibiogram 
of the MRSA isolates revealed the higher resistance to antibiotics as compared to 
methicillin‑sensitive Staphylococcus aureus. All the MRSA isolates were sensitive to linezolid.
Conclusion: HCWs in our hospital showed high nasal carriage rate of MRSA, particularly 
the nursing orderlies which is statistically significant. It is advisable to detect mupirocin 
resistance among the isolates obtained from the HCWs so that in case of resistance, 
alternative treatment should be sought.


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